Interaction in vitro; bait produced from transfected construct; produced from transfected construct.
Bait and prey produced from transfected constructs in S2 cells.
Interaction is not affected by the presence of mutated human genes HECT(C2854A), PABC(Y2509A+C2527A), or UBR(C1272A+C1274A).
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.