Source was larval wing discs; bait produced from endogenous gene; prey produced from endogenous gene.

Loss of mir-9a leads to ectopic expression of sens protein.

Source was larval wing discs; bait produced from transgenic construct; prey produced from endogenous gene.

Overexpression of mir-9a leads to reduction of sens protein level.

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

luciferase reporter assay

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Biotinylated miRNA was transfected, allowed to assemble into RISC complexes, then used to pull down associated mRNA. Positive control.