Arl2
TAP tag
αTub84B
Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
TAP tag was added to Arl2 C-terminus and driven using elav-Gal4.
Venus tag
α-tub
Myc tag
T30N, 3-fold higher binding
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
The GDP-bound form of Arl2 (T30N) binds αTub84B and βTub56D tubulin more strongly than the wild-type or GTP-bound (Q70L) form.
