Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Author\'s reference to GST-labeled protein production leads to a complex trail of reference publications. It appears as if GST-tagged protein was produced by in vitro translation (as opposed to a bacterial system) but it cannot be stated with complete certainty.
Interaction in vitro; bait produced by in vitro translation; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced by in vitro translation; prey produced by endogenous gene.
Interaction in vitro; bait produced by in vitro translation; prey produced and labeled by in vitro translation.
