FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
nanos, Not3
FlyBase ID
FBig0000104684
Interaction Network
Interactions Browser links
nanos network
Not3 network
Reported Interactions
FBrf0232180-10.PD.MW
Reference
Raisch et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

Nanos

bait

GST tag

nanos 
nanos 
Not3 

NOT3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
NOT module binding region
sufficient binding region
aa 116-163

"NBR", coordinates relative to nos-PB

nanos 
NOT module binding region
necessary binding region
aa 116-163

"NBR", coordinates relative to nos-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Raisch et al., 2016)

nos binds only preassembled Not1-Rga-Not3 complex, and does not bind any single Not subunit individually.

(Raisch et al., 2016)
FBrf0232180-3.coIP.WB
Reference
Raisch et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
nanos 

Nanos

bait

GFP tag

nanos 
nanos 
Not3 

NOT3

prey

HA tag

Not3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
Nanos effector domain
sufficient binding region
aa 50-236

coordinates relative to nos-PB

nanos 
Nanos effector domain
necessary binding region
aa 50-236

coordinates relative to nos-PB

Not3 
C-terminal region
sufficient binding region
aa 677-844

coordinates relative to Not3-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Raisch et al., 2016)

The nos-Not3 interaction is RNA-independent.

(Raisch et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)