In vitro synthesized miRNAs were injected into embryos before cellularization, and effects on the reporter were assessed at 10 hr AEL.

Luciferase reporter assay.

2\'O-methyl oligoribonucleotides antisense to mir-11 leads to moderate derepression of the rpr luciferase reporter.

Source was embryos of transgenic fly line; bait synthesized in vitro; prey produced from transgenic reporter construct.

rpr and hid (not skl or grim) transcript levels are elevated in mir-11 mutant background.

Source was third instar larval eye disc; bait produced from endogenous gene; prey produced from endogenous gene.

Overexpression of mir-11 in S2R+ cells repressed hid and rpr transcript levels by about 25%.

Source was S2R+ cells; bait produced from transgenic construct; prey produced from endogenous gene.

HeLa cells were used since S2 cells endogenously express mir-11.

luciferase reporter assay

Source was HeLa cell line; bait produced from transfected construct; prey produced from transfected reporter construct.