Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000104755
Interaction Network
Interactions Browser links
Ube3a network
tkv network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0232477-1.coIP.WB
Reference
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tkv 

Tkv

bait

YFP tag

Ube3a

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval brains of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0232477-2.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Ube3a

enzyme

GST tag

Ube3a

enzyme

GST tag

tkv 

Tkv

enzyme target

Myc tag

tkv 

Tkv

enzyme target

Myc tag

tkv 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0232477-3.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tkv 

Tkv

bait

Myc tag

Ube3a

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

tkv, Ube3a and S6KL were co-transfected, then immunoprecipitated sequentially by Myc then HA tags.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)