FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-1, Nedd4
FlyBase ID
FBig0000104800
Interaction Network
Interactions Browser links
mir-1 network
Nedd4 network
Reported Interactions
FBrf0202410-11.miR-mRNA.LUC
Reference
Easow et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mir-1 

mir-1

bait
Nedd4 

Nedd4

prey
Nedd4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nedd4 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Luciferase reporter assay.

(Easow et al., 2007)

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Easow et al., 2007)
FBrf0202410-4.miR-mRNA.WB
Reference
Easow et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-1 

mir-1

bait
Nedd4 

Nedd4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Mutation of mir-1 increases Nedd4 protein levels.

(Easow et al., 2007)

Source was embryos of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Easow et al., 2007)
FBrf0234934-1.miR-mRNA.LUC
Reference
Zhu et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-1 

miR-1

bait
Nedd4 

Nedd4

prey
Nedd4 
Nedd4 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nedd4 
3-prime UTR
sufficient binding region
Nedd4 
mir-1 target site
necessary binding region
ACAUUCC
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was murine myoblast C2C12 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Zhu et al., 2017)

Luciferase reporter assay.

(Zhu et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)