FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000104863
Interaction Network
Interactions Browser links
mir-277 network
Drep2 network
Reported Interactions
FBrf0218227-1.miR-mRNA.LUC
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-277

bait

Drep2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
3-prime UTR
sufficient binding region
mir-277 target site 1
necessary binding region
AGAGCAGUUUUUGUAGCAUUUAU
mir-277 target site 2
necessary binding region
CUUUGUUAAUCUUGUUGCAUUUC
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Luciferase reporter assay.

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

FBrf0218227-3.miR-mRNA.PCR
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-277

bait

Drep2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Overexpression of mir-277 reduces Drep2 mRNA levels, while expression of a mir-277 sponge increases Drep2 mRNA levels.

Source was adult heads of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)