FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Wdr24, mio
FlyBase ID
FBig0000104876
Interaction Network
Interactions Browser links
Wdr24 network
mio network
Reported Interactions
FBrf0232362-2.coIP.WB
Reference
Cai et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Wdr24 

Wdr24

bait

GFP tag

mio 

Mio

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Cai et al., 2016)
FBrf0232362-8.coIP.MS
Reference
Cai et al., 2016
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mio 

Mio

bait

HA tag, FLAG tag

Wdr24 

Wdr24

prey

7 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

FLAG-HA tandem affinity purification.

(Cai et al., 2016)

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Cai et al., 2016)
FBrf0259115-14.coIP.WB
Reference
Liu et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Wdr24 

WDR24

bait

FLAG tag

mio 

Mio

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2024)
FBrf0259115-17.coIP.WB
Reference
Liu et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mio 

Mio

bait

Myc tag

Wdr24 

WDR24

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Liu et al., 2024)

Cells lacking human GATOR2 component Mios; transfected with Drosophila GATOR2 complex components Wdr59, Sec13 and Nup44A.

(Liu et al., 2024)
FBrf0259115-2.coIP.MS
Reference
Liu et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
mio 

Mio

bait

HA tag

Wdr24 

WDR24

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced endogenous gene.

(Liu et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)