Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult ovarian cDNA expression library).
Two-hybrid system: yeast LexA-BD
Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.