His pull down.

spri interacts with the dominant negative form of Rab5, not the constitutively active form. spri binds Rab5 but not Rab7 or Rab11.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

spri binds a dominant-negative form of Rab5 more strongly than wild-type or constitutively-active forms.