FB2026_02 , released June 18, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Gαo, RapGAP1
FlyBase ID
FBig0000104913
Interaction Network
Interactions Browser links
Gαo network
RapGAP1 network
Reported Interactions
FBrf0209114-3.Y2H
Reference
Kopein and Katanaev, 2009
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Gαo 

Gαo

bait

fused to LexA DNA-binding domain

RapGAP1 

Rapgap1

prey

fused to GAL4 activation domain

RapGAP1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RapGAP1 
GoLoco domain
sufficient binding region
aa 58-166
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Kopein and Katanaev, 2009)

11 RapGAP1 clones recovered in the Gαo screen.

(Kopein and Katanaev, 2009)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult head cDNA expression library).

(Kopein and Katanaev, 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)