FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
usp, foxo
FlyBase ID
FBig0000104999
Interaction Network
Interactions Browser links
usp network
foxo network
Reported Interactions
FBrf0226858-1.PD.WB
Reference
Koyama et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
foxo 

FoxO

bait

GST tag

foxo 
foxo 
foxo 
foxo 
usp 

Usp

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
foxo 
usp interaction domain residue W172
mutation decreasing interaction
aa 172

W172A, coordinates relative to foxo-PB

foxo 
usp interaction domain residue N175
mutation decreasing interaction
aa 175

N175A, coordinates relative to foxo-PB

foxo 
usp interaction domain residue R202
mutation decreasing interaction
aa 202

R202A, coordinates relative to foxo-PB

foxo 
usp interaction domain residue K204
mutation decreasing interaction
aa 204

K204A, coordinates relative to foxo-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system.

(Koyama et al., 2014)

foxo binds usp but not EcR (even in the presence of ecdysone).

(Koyama et al., 2014)

While the single foxo point mutations studied decrease binding only mildly, double mutant combinations abolish binding of foxo to usp.

(Koyama et al., 2014)
FBrf0226858-2.coIP.WB
Reference
Koyama et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
usp 

Usp

bait
foxo 

FoxO

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Koyama et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)