FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
smo, gish
FlyBase ID
FBig0000105000
Interaction Network
Interactions Browser links
smo network
gish network
Reported Interactions
FBrf0232603-1.coIP.WB
Reference
Li et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

smo 
smo 
gish 

Gish

prey

HA tag

gish 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gish 
palmitoylation signal
necessary binding region
SRCCCFFKR

"ΔC" construct

smo 
C-terminal region
sufficient binding region
smo 
membrane proximal region
necessary binding region
aa 570-650
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2016)

The smo-gish interaction is enhanced upon incubation of cells in hh-conditioned medium.

(Li et al., 2016)
FBrf0232603-2.coIP.WB
Reference
Li et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

Myc tag

gish 

Gish

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The smo-gish interaction is enhanced upon incubation of cells in hh-conditioned medium.

(Li et al., 2016)

Source was cell extract of S2 cell line; bait produced from endogenous gene.

(Li et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)