kto, ci Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

kto

Kto

bait

Myc tag

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mao et al., 2014)

FBrf0225834-2.coIP.WB

Reference

Mao et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

bait

FLAG tag

kto

Kto

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mao et al., 2014)

FBrf0225834-6.coIP.WB

Reference

Mao et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

kto

Kto

bait

FLAG tag

kto

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

kto

N-terminal region 1

sufficient binding region

aa 1-289

"a" construct, coordinates relative to kto-PA

kto

N-terminal region 2

sufficient binding region

aa 290-740

"b1" construct, coordinates relative to kto-PA

kto

N-terminal region 3

sufficient binding region

aa 741-1309

"b2" construct, coordinates relative to kto-PA

N-terminal region

sufficient binding region

aa 1-440

"CiN" construct, coordinates relative to ci-PA

central region

sufficient binding region

aa 440-1160

"CiM" construct, coordinates relative to ci-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mao et al., 2014)

Multiple independent regions of kto and ci can mediate the interaction.

(Mao et al., 2014)

FBrf0225834-7.coIP.WB

Reference

Mao et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used