FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ds, pk
FlyBase ID
FBig0000105026
Interaction Network
Interactions Browser links
ds network
pk network
Reported Interactions
FBrf0225692-4.coIP.WB
Reference
Ayukawa et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ds 

Ds

bait

FLAG tag

ds 
pk 

Sple

prey

Myc tag

pk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pk 
Sple-specific N-terminal domain
sufficient binding region
aa 1-130

coordinates relative to pk-PC

ds 
intracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ayukawa et al., 2014)
FBrf0231037-2.coIP.WB
Reference
Ambegaonkar and Irvine, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ds 

Ds

bait

V5 tag

ds 
pk 

Sple

prey

FLAG tag

pk 
pk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ds 
Intracellular domain
sufficient binding region
pk 
N terminal
sufficient binding region
aa 1-349
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ambegaonkar and Irvine, 2015)
FBrf0260993-3.coIP.WB
Reference
Tripathi and Irvine, 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ds 

ds

bait

V5 tag

ds 
pk 

sple

prey

FLAG tag

pk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ds 
intracellular D II and III motif
necessary binding region
aa 246-292

coordinates relative to ds-PA

pk 
N-terminal domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tripathi and Irvine, 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)