Mutation of mir-124 increases sax mRNA levels.

The nervous system was labeled using mir-124 :DsRed reporter in wild-type or mir-124 mutant backgrounds, and labeled cells were isolated from dissociated embryos by FACS.

Source was embryonic central nervous system of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

Luciferase reporter assay.

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.