FB2026_02 , released June 18, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
mir-8, Gug
FlyBase ID
FBig0000105158
Interaction Network
Interactions Browser links
mir-8 network
Gug network
Reported Interactions
FBrf0200486-1.miR-mRNA.PCR
Reference
Karres et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / prepupal stage P4
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-8 

miR-8

bait
Gug 

atrophin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was prepupae of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Karres et al., 2007)

Mutation of mir-8 increases Gug mRNA levels.

(Karres et al., 2007)
FBrf0200486-2.miR-mRNA.LUC
Reference
Karres et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mir-8 

mir-8

bait
Gug 

Atro

prey
Gug 
Gug 
Gug 
Gug 
Gug 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Gug 
3-prime UTR
sufficient binding region
Gug 
mir-8 target site 1
necessary binding region
AAUAUCUUAAGUAUCCUCUUCAUAGUGUAUAGUGUUAA

conserved in D. pseudoobscura

Gug 
mir-8 target site 2
necessary binding region
CAUAUAUAUAUUCGAGAGUAUUGU
Gug 
mir-8 target site 3
necessary binding region
AUUUUGCUAUGACUAGAGUAUUAU

conserved in D. pseudoobscura

Gug 
mir-8 target site 4
necessary binding region
AUUAUAUGGGUAUUAU
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Karres et al., 2007)

Luciferase reporter assay.

(Karres et al., 2007)
FBrf0200486-6.miR-mRNA.PCR
Reference
Karres et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / prepupal stage P4
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-8 

miR-8

bait
Gug 

Atro

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was prepupae of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Karres et al., 2007)

Mutation of mir-8 increases Gug mRNA levels.

(Karres et al., 2007)

Microarray expression data.

(Karres et al., 2007)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)