Source was adult males; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.
The roX2 bait RNA fused to three MS2 hairpins, and bound to amylose beads via MS2-MBP fusion protein.
Interaction in vitro; bait produced by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.
Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; RNA chemically synthesized.
