FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
smo, Pka-C1
FlyBase ID
FBig0000105211
Interaction Network
Interactions Browser links
smo network
Pka-C1 network
Reported Interactions
FBrf0225554-1.coIP.WB
Reference
Li et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
smo 

smo

bait

Myc tag

smo 
Pka-C1 

Pka-C1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal domain
mutation disrupting interaction
aa 667,687,740aa 593-595,645-647

S667A,S687A,S740A

K593RK to AAA and K645RR to AAA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Li et al., 2014)
FBrf0225554-2.coIP.WB
Reference
Li et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
smo 

smo

bait

Myc tag

smo 
Pka-C1 

Pka-C1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
C-terminal domain
mutation disrupting interaction
aa 667,687,740

S667A,S687A,S740A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of CME-W1-Cl.8+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Li et al., 2014)
FBrf0226417-15.coIP.WB
Reference
Ranieri et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Pka-C1 

PKA

bait

HA tag

smo 

Smo

prey

Myc tag

smo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
PKA sites
sufficient binding region
aa 581-798
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ranieri et al., 2014)

smo outcompetes ci for binding to Pka-C1 in this assay.

(Ranieri et al., 2014)
FBrf0226417-17.BIFC.FM
Reference
Ranieri et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

neutral component

YFP tag, N-terminal fragment

smo 

Smo

neutral component

YFP tag, N-terminal fragment

smo 

Smo

neutral component

YFP tag, N-terminal fragment

Pka-C1 

PKA

neutral component

YFP tag, C-terminal fragment

Pka-C1 

PKA

neutral component

YFP tag, C-terminal fragment

Pka-C1 

PKA

neutral component

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2R+ cells; proteins produced from transfected constructs.

(Ranieri et al., 2014)
FBrf0226417-6.coIP.WB
Reference
Ranieri et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
CME-W1-Cl.8+
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait
Pka-C1 

PKA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of CME-W1-Cl.8+ cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Ranieri et al., 2014)
FBrf0235777-6.coIP.WB
Reference
Chen et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
smo 

smo

bait

Myc tag

Pka-C1 

Pka-C1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Chen et al., 2017)
FBrf0243776-3.coIP.WB
Reference
Fan et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
smo 

Smo

bait

FLAG tag

Pka-C1 

PKAc

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fan et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)