Two-hybrid system: yeast LexA-BD/AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

64 of 185 Cad96Ca-interacting clones represented drk.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

drk does not interact with kinase-dead Cad96Ca.