FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cep135, ana2
FlyBase ID
FBig0000105331
Interaction Network
Interactions Browser links
Cep135 network
ana2 network
Reported Interactions
FBrf0226888-6.PD.A
Reference
Dzhindzhev et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ana2 

Ana2

bait

GST tag

Cep135 

Bld10

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The SAK-phosphorylated form of ana2 binds to Cep135 3.5-fold more strongly than the non-phosphorylated form of ana2.

(Dzhindzhev et al., 2014)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Dzhindzhev et al., 2014)

ana2 was treated with functional or kinase-dead SAK to assess the effects of SAK phosphorylation on ana2 binding of other proteins.

(Dzhindzhev et al., 2014)
FBrf0233282-32.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cep135 

CEP135/Bld10

bait

fused to GAL4 DNA-binding domain

Cep135 
ana2 

Ana2

prey

fused to GAL4 activation domain

ana2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ana2 
C-terminal region
sufficient binding region
aa 177-420

coordinates relative to ana2-PA

Cep135 
N-terminal region
sufficient binding region
aa 1-490

coordinates relative to Cep135-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)

Weak interaction.

(Galletta et al., 2016)
FBrf0246771-2.coIP.WB
Reference
McLamarrah et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DRSC
Participants
Corresponds to
Reported as
Role
Note
Cep135 

CEP135

bait

GFP tag

ana2 

Ana2

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(McLamarrah et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)