FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
kst, kibra
FlyBase ID
FBig0000105407
Interaction Network
Interactions Browser links
kst network
kibra network
Reported Interactions
FBrf0227970-3.coIP.WB
Reference
Fletcher et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kst 

βH-Spec

bait

GFP tag

kibra 

Kibra

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Fletcher et al., 2015)
FBrf0227970-6.coIP.WB
Reference
Fletcher et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
kst 

βH-Spec

bait

FLAG tag

kst 
kibra 

Kibra

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kst 
N-terminal region
sufficient binding region
aa 1-1632

coordinates relative to kst-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Fletcher et al., 2015)

kibra coimmunoprecipitates with the N-terminal region of kst, and more weakly with other regions of kst.

(Fletcher et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)