FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
kay, chm
FlyBase ID
FBig0000105441
Interaction Network
Interactions Browser links
kay network
chm network
Reported Interactions
FBrf0190553-2.PD.WB
Reference
Miotto et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kay 

DFos

bait

GST tag

kay 
kay 
chm 

Chm

prey

His tag

chm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kay 
bZIP domain
sufficient binding region

major binding region

kay 
C-terminal region
sufficient binding region

minor binding region

chm 
C-terminal region
sufficient binding region
aa 494-811

coordinates relative to chm-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Miotto et al., 2006)
FBrf0190553-3.coIP.WB
Reference
Miotto et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
chm 

Chm

bait

Myc tag

kay 

DFos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Miotto et al., 2006)
FBrf0190553-5.coIP.WB
Reference
Miotto et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kay 

DFos

bait

TAP tag

chm 

Chm

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Miotto et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)