piwi, His1 Physical Interaction report

peptide massfingerprinting

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

piwi

Piwi

bait

His1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Iwasaki et al., 2016)

FBrf0233069-2.coIP.WB

Reference

Iwasaki et al., 2016

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

piwi

Piwi

bait

His1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Iwasaki et al., 2016)

piwi immunoprecipitates His1 but not His3, His2A or Su(var)205. This interaction is RNA- and DNA-independent.

(Iwasaki et al., 2016)

FBrf0233069-3.coIP.WB

Reference

Iwasaki et al., 2016

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

His1

bait

piwi

Piwi

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was nuclear extract of OSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Iwasaki et al., 2016)

His1 immunoprecipitates piwi but not mael or arx. This interaction is RNA- and DNA-independent.

(Iwasaki et al., 2016)

FBrf0233069-4.coIP.WB

Reference

Iwasaki et al., 2016

Description

physical association

Assay

Collection

Source/Stage

ovary / female adult stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

piwi

Piwi

bait

His1

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Iwasaki et al., 2016)

FBrf0233069-5.PD.WB

Reference

Iwasaki et al., 2016

Description

physical association

Assay

pull down

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

His1

bait

GST tag

His1

piwi

Piwi

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

His1

C-terminal region

sufficient binding region

aa 40-256

"ΔN" construct

His1

C-terminal region

necessary binding region

aa 131-256

"ΔC" construct

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from OSC cell extract.

(Iwasaki et al., 2016)

FBrf0233069-6.PD.WB

Reference

Iwasaki et al., 2016

Description

physical association

Assay

pull down

anti tag western blot

Collection

Source/Stage

Cell line used