FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
yki, Kap-α1
FlyBase ID
FBig0000105514
Interaction Network
Interactions Browser links
yki network
Kap-α1 network
Reported Interactions
FBrf0233262-3.PD.MS
Reference
Wang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

GST tag

yki 
Kap-α1 

Importin-alpha1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
N-terminal region
sufficient binding region
MCACLIAKIILCSFRLYTISAFYMLTTMSASSNTNSLIEKEIDDEDMLSPIKSNN
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Wang et al., 2016)
FBrf0233262-4.coIP.WB
Reference
Wang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
yki 

Yki

bait

Myc tag

yki 
yki 
yki 
Kap-α1 

Importin-alpha1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
N-terminal region
sufficient binding region
MCACLIAKIILCSFRLYTISAFYMLTTMSASSNTNSLIEKEIDDEDMLSPIKSNN
yki 
N-terminal region
necessary binding region
MCACLIAKIILCSFRLYTISAFYMLTTMSASSNTNSLIEKEIDDEDMLSPIKSNN
yki 
N-terminal region residue 15
mutation disrupting interaction
aa 15

R15A (FRLY to FALY), coordinates relative to yki-PH

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2016)

yki interacts with Kap-α1 but not with Pen (Kap-α2) or Kap-α3 in this assay.

(Wang et al., 2016)
FBrf0233262-5.coIP.WB
Reference
Wang et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Kap-α1 

α1

bait

FLAG tag

yki 

Yki

prey

Myc tag

yki 
yki 
yki 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
yki 
N-terminal region
sufficient binding region
MCACLIAKIILCSFRLYTISAFYMLTTMSASSNTNSLIEKEIDDEDMLSPIKSNN
yki 
N-terminal region
necessary binding region
MCACLIAKIILCSFRLYTISAFYMLTTMSASSNTNSLIEKEIDDEDMLSPIKSNN
yki 
N-terminal region residue 15
mutation disrupting interaction
aa 15

R15A (FRLY to FALY), coordinates relative to yki-PH

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wang et al., 2016)

yki interacts with Kap-α1 but not with Pen (Kap-α2) or Kap-α3 in this assay.

(Wang et al., 2016)

The yki-Kap-α1 interaction is attenuated upon cotransfection of hpo or GTP-bound Ran (Q69L).

(Wang et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)