FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
milt, Khc
FlyBase ID
FBig0000105529
Interaction Network
Interactions Browser links
milt network
Khc network
Reported Interactions
FBrf0156010-1.coIP.WB
Reference
Stowers et al., 2002
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
milt 

Milton

bait
Khc 

KHC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Stowers et al., 2002)
FBrf0156010-2.coIP.WB
Reference
Stowers et al., 2002
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Khc 

KHC

bait
milt 

Milton

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Stowers et al., 2002)
FBrf0194133-1.coIP.WB
Reference
Glater et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
milt 

Milt

bait
milt 
Khc 

KHC

prey

MYC tag

Khc 
Khc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
milt 
N terminal domain with coiled coil motif
sufficient binding region
aa 1-450

coordinates relative to milt-PA

Khc 
N terminal region
sufficient binding region
aa 1-891
Khc 
COOH tail region
necessary binding region
aa 810-891
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Glater et al., 2006)

milt-Khc interaction is inhibited by coexpression of Klc

(Glater et al., 2006)

milt isoform C, corresponding to milt-PD, does not interact with Khc in this assay.

(Glater et al., 2006)
FBrf0194133-2.coIP.WB
Reference
Glater et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Khc 

KHC

bait

MYC tag

milt 

Milt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

milt-Khc interaction is inhibited by coexpression of Klc

(Glater et al., 2006)

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Glater et al., 2006)
FBrf0194133-4.coIP.WB
Reference
Glater et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
milt 

milt

bait
Khc 

Khc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Glater et al., 2006)
FBrf0194133-6.coIP.WB
Reference
Glater et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Khc 

Khc

bait
milt 

milt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Glater et al., 2006)
FBrf0233299-2.coIP.WB
Reference
Winding et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Khc 

KHC

bait

GFP tag

Khc 
milt 

Milton

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Khc 
tail
sufficient binding region
aa 345-975

coordinates relative to Khc-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Winding et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)