FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Z600, CycE
FlyBase ID
FBig0000105574
Interaction Network
Interactions Browser links
Z600 network
CycE network
Reported Interactions
FBrf0202415-13.Y2H
Reference
Gawlinski et al., 2007
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Z600 

Frs

bait

fused to LexA DNA-binding domain

Z600 
CycE 

CycE

prey

fused to activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Z600 
KxL motif
necessary binding region
RKSLNF
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).

(Gawlinski et al., 2007)

Two-hybrid system: yeast LexA-BD

(Gawlinski et al., 2007)
FBrf0202415-14.MS
Reference
Gawlinski et al., 2007
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Z600 

Frs

bait
CycE 

Imp7

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gawlinski et al., 2007)
FBrf0202415-18.SPR
Reference
Gawlinski et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Z600 

Frs

bait

His tag

CycE 

CycE

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Gawlinski et al., 2007)

Z600 binds CycE with Kd = 96 nM.

(Gawlinski et al., 2007)
FBrf0202415-4.PD.A
Reference
Gawlinski et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Z600 

frs

bait

GST tag

CycE 

CycE

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Gawlinski et al., 2007)

Z600 binds to various cyclins, but not to Cdk1 in vitro.

(Gawlinski et al., 2007)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)