Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.
Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
I267A,L271A, coordinates relative to Tm1-PC
L271A or L271R, coordinates relative to Tm1-PC
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.
I267A,L271A, coordinates relative to Tm1-PC
L271A, coordinates relative to Tm1-PC, Kd = 3.89uM
Kd = 141nM
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.
