FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dsh, dlg1
FlyBase ID
FBig0000106079
Interaction Network
Interactions Browser links
dsh network
dlg1 network
Reported Interactions
FBrf0226961-1.PD.WB
Reference
Garcia et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dsh 

Dsh

bait

GST tag

dsh 
dlg1 

Dlg

prey

His tag

dlg1 
dlg1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dsh 
PDZ-DEP domains
sufficient binding region
aa 260-623

coordinates relative to dsh-PA

dlg1 
C-terminal region
sufficient binding region
aa 598-975

coordinates relative to dlg1-PG

dlg1 
Hook domain
necessary binding region
aa 679-770

"ΔHook" construct, coordinates relative to dlg1-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vit o; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Garcia et al., 2014)
FBrf0226961-2.PD.WB
Reference
Garcia et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dlg1 

Dlg

bait

GST tag

dlg1 
dlg1 
dlg1 
dlg1 
dlg1 
dsh 

Dsh

prey

His tag

dsh 
dsh 
dsh 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dsh 
PDZ domain
sufficient binding region
aa 260-340

coordinates relative to dsh-PA

dsh 
PDZ domain residue D319
mutation disrupting interaction
aa 319

D319A, coordinates relative to dsh-PA

dsh 
PDZ domain residue L325
mutation disrupting interaction
aa 325

L325A, coordinates relative to dsh-PA

dlg1 
Hook domain I3-insert
sufficient binding region
aa 679-712

coordinates relative to dlg1-PG

dlg1 
Hook domain I3-insert residue K699
mutation disrupting interaction
aa 699

K699A, coordinates relative to dlg1-PG

dlg1 
Hook domain I3-insert residue K700
mutation disrupting interaction
aa 700

K700A, coordinates relative to dlg1-PG

dlg1 
Hook domain I3-insert residue F704
mutation disrupting interaction
aa 704

F704A, coordinates relative to dlg1-PG

dlg1 
Hook domain I3-insert residue F708
mutation disrupting interaction
aa 708

F708A, coordinates relative to dlg1-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Garcia et al., 2014)
FBrf0233376-1.coIP.WB
Reference
Liu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
dsh 

Dsh

bait

Myc tag

dlg1 

Dlg1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Liu et al., 2016)
FBrf0233376-2.coIP.WB
Reference
Liu et al., 2016
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dsh 

Dsh

bait

GFP tag

dlg1 

Dlg1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Liu et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)