FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
so, Opbp
FlyBase ID
FBig0000106535
Interaction Network
Interactions Browser links
so network
Opbp network
Reported Interactions
FBrf0190132-4.Y2H
Reference
Kenyon et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
so 

SO

bait

fused to GAL4 DNA-binding domain

so 
so 
Opbp 

Obp

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
so 
Six and Homeobox domains
sufficient binding region

"SD+HD" construct, coordinates not reported

so 
Six domain residue V200
mutation increasing interaction
aa 200

V200D, coordinates relative to so-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Kenyon et al., 2005)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kenyon et al., 2005)
FBrf0190132-5.coIP.A
Reference
Kenyon et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Opbp 

Obp

bait

HA tag

so 

SO

prey

[35]S label

so 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
so 
Six domain residue V200
mutation increasing interaction
aa 200

V200D, coordinates relative to so-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from transfected S2 cell extract; prey produced and labeled by in vitro translation.

(Kenyon et al., 2005)
FBrf0190234-11.PD.A
Reference
Kenyon et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
so 

SO

bait

GST tag

so 
Opbp 

Obp

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
so 
SD and HD domains
sufficient binding region
aa 103-213 and 224-274

coordinates relative to so-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kenyon et al., 2005)

weak interaction

(Kenyon et al., 2005)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)