Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Higher molecular weight species of dl detected by both anti-FLAG and anti-HA antibodies indicate the existence of dl covalently conjugated to smt3.
Cells were transfected with FLAG-dl, cact, lwr (Ubc9) and HA-smt3, and protein extracts were analyzed by SDS-PAGE and western blot.