FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
crb, ebi
FlyBase ID
FBig0000106600
Interaction Network
Interactions Browser links
crb network
ebi network
Reported Interactions
FBrf0233653-1.Y2H
Reference
Nguyen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

fused to GAL4 DNA-binding domain

crb 
ebi 

Ebi

prey

fused to GAL4 activation domain

ebi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
fourth laminin domain
sufficient binding region
aa 1576-1792

coordinates relative to crb-PA

ebi 
WD40 repeats 7 and 8
sufficient binding region
aa 614-696

coordinates relative to ebi-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Nguyen et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult cDNA expression library).

(Nguyen et al., 2016)
FBrf0233653-2.coIP.WB
Reference
Nguyen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

V5 tag

crb 
ebi 

Ebi

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
fourth laminin and transmembrane domains
sufficient binding region
aa 1576-2106

coordinates relative to crb-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Nguyen et al., 2016)
FBrf0233653-3.PD.A
Reference
Nguyen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ebi 

Ebi

bait

GST tag

ebi 
ebi 
crb 

Crb

prey

MBP tag

crb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
fourth laminin domain
sufficient binding region
aa 1576-1792

coordinates relative to crb-PA

ebi 
WD40 repeats 7 and 8
sufficient binding region
aa 614-696

coordinates relative to ebi-PA

ebi 
WD40 repeats 7 and 8
necessary binding region
aa 614-696

coordinates relative to ebi-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Nguyen et al., 2016)

Of all regions tested, only the fourth laminin domain of crb binds to ebi.

(Nguyen et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)