FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
imd, pirk
FlyBase ID
FBig0000106612
Interaction Network
Interactions Browser links
imd network
pirk network
Reported Interactions
FBrf0205806-4.coIP.WB
Reference
Aggarwal et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
imd 

IMD

bait

FLAG tag

pirk 

Rdr

prey

T7 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2* or HEK cell line; bait produced from transfected construct; prey produced from transfected construct.

(Aggarwal et al., 2008)
FBrf0205850-3.coIP.WB
Reference
Lhocine et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
imd 

Imd

bait

HA tag

pirk 

PIMS

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lhocine et al., 2008)
FBrf0206659-2.coIP.WB
Reference
Kleino et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
imd 

Imd

bait

Myc tag

pirk 

Pirk

prey

V5 tag

pirk 
pirk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pirk 
Pirk domain
sufficient binding region
aa 52-136

coordinates relative to pirk-PA

pirk 
C-terminal region
sufficient binding region
aa 137-197

weak binding, coordinates relative to pirk-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kleino et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)