FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Atg8a, ref(2)P
FlyBase ID
FBig0000106679
Interaction Network
Interactions Browser links
Atg8a network
ref(2)P network
Reported Interactions
FBrf0225216-7.coIP.WB
Reference
Hegedűs et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Atg8a 

Atg8a

bait

HA tag

ref(2)P 

ref(2)P

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hegedűs et al., 2014)
FBrf0228687-1.PD.A
Reference
Jain et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atg8a 

DmAtg8a

bait

GST tag

Atg8a 
Atg8a 
ref(2)P 

Ref(2)P

prey

[35]S label

ref(2)P 
ref(2)P 
ref(2)P 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ref(2)P 
LIR motif
necessary binding region
DPEWQLIDN

"ΔLIR" construct

ref(2)P 
LIR motif residue W454
mutation disrupting interaction
aa 454

W454A, coordinates relative to ref(2)P-PA

ref(2)P 
LIR motif residue I457
mutation disrupting interaction
aa 457

I457A, coordinates relative to ref(2)P-PA

Atg8a 
N-terminal region
sufficient binding region
aa 1-71

coordinates relative to Atg8a-PA

Atg8a 
LIR-binding site residues K48 and Y49
mutation disrupting interaction
aa 48-49

K48A, Y49A, coordinates relative to Atg8a-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Jain et al., 2015)
FBrf0228687-2.coIP.WB
Reference
Jain et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
ref(2)P 

Ref(2)P

bait

FLAG tag

ref(2)P 
ref(2)P 
ref(2)P 
Atg8a 

DmAtg8a

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ref(2)P 
LIR motif
necessary binding region
DPEWQLIDN

"ΔLIR" construct

ref(2)P 
LIR motif residue W454
mutation disrupting interaction
aa 454

W454A, coordinates relative to ref(2)P-PA

ref(2)P 
LIR motif residue I457
mutation disrupting interaction
aa 457

I457A, coordinates relative to ref(2)P-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jain et al., 2015)
FBrf0254596-1.coIP.WB
Reference
Bhattacharjee et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atg8a 

Atg8a

bait

mCherry tag

ref(2)P 

ref(2)P

prey
ref(2)P 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ref(2)P 
LIR domain residues 454 and 457
necessary binding region
aa 454 and 457

W454A, I457A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was flies from transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bhattacharjee et al., 2022)
FBrf0262785-2.PLA
Reference
Katz et al., 2025
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval lymph gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atg8a 

Atg8a

neutral component
Atg8a 

Atg8a

neutral component
Atg8a 

Atg8a

neutral component
ref(2)P 

Ref(2)P

neutral component

GFP tag

ref(2)P 

Ref(2)P

neutral component

GFP tag

ref(2)P 

Ref(2)P

neutral component

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval lymph glands of transgenic fly line. Atg8a produced from endogenous gene; ref(2)P produced from transgenic construct.

(Katz et al., 2025)

Positive control.

(Katz et al., 2025)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)