FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Orai, Stim
FlyBase ID
FBig0000106815
Interaction Network
Interactions Browser links
Orai network
Stim network
Reported Interactions
FBrf0204959-1.coIP.WB
Reference
Yeromin et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Orai 

olf186-F

bait

HA tag

Stim 

Stim

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yeromin et al., 2006)

After calcium store depletion by thapsigargin treatment, the olf186-F-Stim interaction is enhanced.

(Yeromin et al., 2006)
FBrf0204959-2.coIP.WB
Reference
Yeromin et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Stim 

Stim

bait

V5 tag

Orai 

olf186-F

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yeromin et al., 2006)

After calcium store depletion by thapsigargin treatment, the olf186-F-Stim interaction is enhanced.

(Yeromin et al., 2006)
FBrf0233736-1.coIP.WB
Reference
Chakraborty et al., 2016
Description
physical association
Assay
Collection
Source/Stage
larval brain / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Orai 

Orai

bait

CFP tag

Stim 

STIM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval brains of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Chakraborty et al., 2016)
FBrf0233736-2.coIP.WB
Reference
Chakraborty et al., 2016
Description
physical association
Assay
Collection
Source/Stage
larval brain / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Stim 

STIM

bait

YFP tag

Orai 

Orai

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The olf186-F-Stim interaction was enhanced upon treatment of larvae with thapsigargin, which depletes Ca++ stores.

(Chakraborty et al., 2016)

Source was larval brains of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Chakraborty et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)