FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
PGRP-LC, pirk
FlyBase ID
FBig0000106875
Interaction Network
Interactions Browser links
PGRP-LC network
pirk network
Reported Interactions
FBrf0205806-1.Y2H
Reference
Aggarwal et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
PGRP-LC 

PGRP-LC

bait

fused to DNA-binding domain

PGRP-LC 
PGRP-LC 
PGRP-LC 
pirk 

rudra

prey

fused to activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PGRP-LC 
cytoplasmic domain
sufficient binding region
aa 1-253

coordinates relative to PGRP-LC-PA

PGRP-LC 
RHIM-like domain
mutation decreasing interaction
aa 172-242

"Δ172-212" and "Δ213-242" constructs, coordinates relative to PGRP-LC-PA

PGRP-LC 
cytoplasmic domain residue F218
mutation decreasing interaction
aa 218

F218A, coordinates relative to PGRP-LC-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast

(Aggarwal et al., 2008)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).

(Aggarwal et al., 2008)
FBrf0205806-3.coIP.WB
Reference
Aggarwal et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
PGRP-LC 

PGRP-LC

bait

V5 tag or T7 tag

PGRP-LC 
PGRP-LC 
pirk 

Rdr

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PGRP-LC 
N-terminal region
sufficient binding region
aa 1-333

coordinates relative to PGRP-LC-PA

PGRP-LC 
N-terminal region
necessary binding region
aa 1-253

coordinates relative to PGRP-LC-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2* cell line; bait produced from transfected construct; prey produced from transfected construct.

(Aggarwal et al., 2008)

Deletion analysis suggests the presence of multiple binding sites in PGRP-LC for pirk.

(Aggarwal et al., 2008)
FBrf0205850-1.PD.WB
Reference
Lhocine et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
pirk 

PIMS

bait

GST tag

PGRP-LC 

PGRP-LC

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lhocine et al., 2008)
FBrf0205850-2.coIP.WB
Reference
Lhocine et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
PGRP-LC 

PGRP-LC

bait

V5 tag

pirk 

PIMS

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Lhocine et al., 2008)
FBrf0206659-1.coIP.WB
Reference
Kleino et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
PGRP-LC 

LC

bait

Myc tag

PGRP-LC 
pirk 

Pirk

prey

V5 tag

pirk 
pirk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PGRP-LC 
cytoplasmic tail
sufficient binding region
aa 1-293

coordinates relative to PGRP-LC-PA

pirk 
C-terminal region
sufficient binding region
aa 137-197

coordinates relative to pirk-PA

pirk 
Pirk domain
sufficient binding region
aa 52-136

weak binding, coordinates relative to pirk-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kleino et al., 2008)
FBrf0233515-1.coIP.WB
Reference
Neyen et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
pirk 

Pirk

bait

HA tag

PGRP-LC 

PGRP-LC

prey

V5 tag

PGRP-LC 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PGRP-LC 
PHD domain
necessary binding region
aa 1-65

"ΔPHD" construct, coordinates relative to PGRP-LC-PE.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Neyen et al., 2016)

Both the N-terminal variants of PGRP-LC bind pirk. Deletion of the PHD domain affects pirk binding by the "regulatory" form of PGRP-LC.

(Neyen et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)