FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
scrib, tkv
FlyBase ID
FBig0000107051
Interaction Network
Interactions Browser links
scrib network
tkv network
Reported Interactions
FBrf0233965-1.coIP.WB
Reference
Gui et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
tkv 

Tkv

bait

GFP tag

scrib 

Scrib

prey

Myc tag

scrib 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
scrib 
LRR domain
sufficient binding region
aa 1-692

"LRR" construct, coordinates relative to scrib-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gui et al., 2016)

Full length scrib weakly associated with tkv, but the scrib LRR domain alone binds tkv strongly.

(Gui et al., 2016)
FBrf0233965-2.coIP.WB
Reference
Gui et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
scrib 

Scrib

bait

RFP tag

scrib 
tkv 

Tkv

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
scrib 
LRR domain
sufficient binding region
aa 1-692

"LRR" construct, coordinates relative to scrib-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gui et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)