Caf1-55, MTA1-like, HDAC1 and MBD-like were coexpressed in a baculovirus expression system, the core NuRD complex affinity purified, and subject to size exclusion chromatography.

Multi-angle laser light scattering of the complex indicated a molecular mass of ~600 kDa.

Source was cell extract of baculovirus-infected Sf21 cell line; bait produced from transfected construct; prey produced from transfected construct.

Caf1-55, MTA1-like, HDAC1 and MBD-like were coexpressed in a baculovirus expression system.

Caf1-55, MTA1-like, HDAC1 and MBD-like constitute the core NuRD complex.

Source was cell extract of baculovirus-infected Sf21 cell line; bait produced from transfected construct; prey produced from transfected construct.

Co-elution of Mi-2, MEP-1, HDAC1, MTA1-like and Caf1-55 in gel filtration captured as all possible binary interactions.

Source was nuclear extract of OSC cell line; bait produced from endogenous gene; prey produced from endogenous gene.