FB2026_02 , released June 18, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
piwi, vas
FlyBase ID
FBig0000107331
Interaction Network
Interactions Browser links
piwi network
vas network
Reported Interactions
FBrf0191897-1.coIP.WB
Reference
Megosh et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
piwi 

piwi

bait

TAP tag

vas 

VASA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Megosh et al., 2006)
FBrf0191897-2.coIP.WB
Reference
Megosh et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

VASA

bait
piwi 

piwi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Megosh et al., 2006)
FBrf0245250-11.coIP.WB
Reference
Venkei et al., 2020
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
piwi 

Piwi

bait

GFP tag

vas 

Vasa

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was testis of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Venkei et al., 2020)

The piwi-vas interaction was detected in testes in which upd1 was also expressed. It was not detected in the absence of mitotic arrest or down-regulation of Klp10A.

(Venkei et al., 2020)
FBrf0257295-13.coIP.WB
Reference
Lin et al., 2023
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

Vas

bait

GFP tag

piwi 

Piwi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Embryos were paraformaldehyde crosslinked before affinity purification; weak interaction.

(Lin et al., 2023)

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Lin et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)