Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic adult extract.
Source was adults of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.
Source was live S2 cells; proteins produced from transfected constructs.
Uses sevS11 allele, a constitutively activated form that consists of the C-terminal Sev protein, which includes the intracellular tyrosine kinase domain, bound to the signal peptide of the Drosophila cuticle protein CP3 and the 10 amino acid Myc epitope. Interaction disrupted by coexpression of Socs36E but not coexpression of Socs36E-R500K
Residue Y2546 phosphorylated; coordinates relative to sev-PA
Interaction in vitro; drk produced as a recombinant fusion protein in bacterial system; sev chemically synthesized.
