Mon1
fused to GAL4 DNA-binding domain
Rab7
fused to GAL4 activation domain
Three-hybrid system: yeast GAL4-BD/GAL4-AD
Ccz1 was coexpressed with Mon1.
The Mon1-Ccz1 complex binds only the GDP-bound (inactivated) variant of Rab7.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
