FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pen-2, aph-1
FlyBase ID
FBig0000107574
Interaction Network
Interactions Browser links
pen-2 network
aph-1 network
Reported Interactions
FBrf0184047-3.coIP.WB
Reference
Niimura et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
aph-1 

Aph-1

bait

FLAG tag

aph-1 
pen-2 

Pen-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
aph-1 
GXXXG motif
mutation disrupting interaction
aa 112aa 112aa 112aa 116aa 116

G112D

G112F

G112L

G116D

G116L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Niimura et al., 2005)
FBrf0211009-10.coIP.WB
Reference
Stempfle et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pen-2 

Pen-2

bait

FLAG tag

aph-1 

Aph-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Stempfle et al., 2010)
FBrf0211009-5.coIP.WB
Reference
Stempfle et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aph-1 

Aph-1

bait
pen-2 

Pen-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Stempfle et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)