FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
brm, Tctp
FlyBase ID
FBig0000107604
Interaction Network
Interactions Browser links
brm network
Tctp network
Reported Interactions
FBrf0233587-1.Y2H
Reference
Hong and Choi, 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tctp 

Tctp

bait

fused to GAL4 DNA-binding domain

brm 

Brahma

prey

fused to GAL4 activation domain

brm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
brm 
interaction domain
sufficient binding region
aa 311-1638

coordinates relative to brm-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Hong and Choi, 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Hong and Choi, 2016)
FBrf0233587-2.coIP.WB
Reference
Hong and Choi, 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
brm 

Brm

bait

FLAG tag

brm 
brm 
Tctp 

Tctp

prey

Myc tag

Tctp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
brm 
HSA domain
mutation decreasing interaction

"ΔHSA" construct

brm 
BRK domain
mutation decreasing interaction

"ΔBRK" construct

Tctp 
residue E12
mutation decreasing interaction
aa 12

E12V, coordinates relative to Tctp-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hong and Choi, 2016)

Deletion of the HSA and BRK domains of brk greatly reduces but does not completely abolish binding to Tctp.

(Hong and Choi, 2016)
FBrf0233587-3.coIP.WB
Reference
Hong and Choi, 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Tctp 

Tctp

bait

Myc tag

brm 

Brm

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hong and Choi, 2016)
FBrf0233587-4.PD.WB
Reference
Hong and Choi, 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brm 

Brm

bait

GST tag

brm 
brm 
brm 
Tctp 

Tctp

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
brm 
central region
sufficient binding region
aa 304-747

coordinates relative to brm-PA

brm 
HSA domain
mutation decreasing interaction

"ΔHSA" construct

brm 
BRK domain
mutation decreasing interaction

"ΔBRK" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Hong and Choi, 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)