FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
eIF4E4, eIF4G1
FlyBase ID
FBig0000107901
Interaction Network
Interactions Browser links
eIF4E4 network
eIF4G1 network
Reported Interactions
FBrf0184136-4.Y2H
Reference
Hernandez et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E4 

4E4

bait

fused to GAL4 DNA-binding domain

eIF4G1 

eIF4G

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Hernandez et al., 2005)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Hernandez et al., 2005)
FBrf0233177-2.FS
Reference
Zuberek et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E4 

eIF4E-4

bait

His tag

eIF4G1 

eIF4G

prey
eIF4G1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4G1 
eIF4E-binding site
sufficient binding region
KKQYDREQLLQLR
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized.

(Zuberek et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)