FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\eIF4E4
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General Information
Symbol
Dmel\eIF4E4
Species
D. melanogaster
Name
eukaryotic translation initiation factor 4E4
Annotation Symbol
CG10124
Feature Type
protein_coding_gene
FlyBase ID
FBgn0035709
Gene Model Status
Current
Stock Availability
62 publicly available
Gene Summary
eukaryotic translation initiation factor 4E4 (eIF4E4) encodes a protein involved in translational initiation. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)
All Summaries
Also Known As

eIF4E-4, eIF4E

Key Links
Genomic Location
Cytogenetic map
65C1-65C1
Sequence location
Recombination map
3-17
RefSeq locus
NT_037436 REGION:6664224..6665641
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
enables eukaryotic initiation factor 4G binding
inferred from physical interaction with FLYBASE:eIF4G1; FB:FBgn0023213
(Hernandez et al., 2005)
enables protein binding
inferred from physical interaction with FLYBASE:Thor; FB:FBgn0261560
(Hernandez et al., 2005)
enables RNA 7-methylguanosine cap binding
inferred from direct assay
(Hernandez et al., 2005)
enables translation initiation factor activity
inferred from genetic interaction with SGD:S000005499
(Hernandez et al., 2005)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables RNA 7-methylguanosine cap binding
inferred from biological aspect of ancestor with PANTHER:PTN000238660
(GO Reference Genome Project, 2011-)
enables RNA binding
inferred from electronic annotation with InterPro:IPR001040, InterPro:IPR019770
(InterPro Project Members, 2004-)
enables RNA cap binding
inferred from sequence or structural similarity
(Lasko, 2000)
enables translation initiation factor activity
inferred from biological aspect of ancestor with PANTHER:PTN000238660
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity
(Lasko, 2000)
inferred from electronic annotation with InterPro:IPR001040, InterPro:IPR019770
(InterPro Project Members, 2004-)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in translational initiation
inferred from genetic interaction with SGD:S000005499
(Hernandez et al., 2005)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in translational initiation
inferred from biological aspect of ancestor with PANTHER:PTN000238660
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR001040, InterPro:IPR019770
(InterPro Project Members, 2004-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
part_of eukaryotic translation initiation factor 4F complex
inferred from physical interaction with FLYBASE:eIF4G1; FB:FBgn0023213
(Hernandez et al., 2005)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from electronic annotation with InterPro:IPR019770
(InterPro Project Members, 2004-)
part_of eukaryotic translation initiation factor 4F complex
inferred from biological aspect of ancestor with PANTHER:PTN000238660
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity
(Lasko, 2000)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
eukaryotic translation initiation factor 4E4 (eIF4E4) encodes a protein involved in translational initiation. [Date last reviewed: 2019-07-11]
Gene Group (FlyBase)
CYTOPLASMIC TRANSLATION INITIATION FACTORS -
Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\eIF4E4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VRY0)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
eIF4E4-RA
FBtr0076963
NM_139795
1031
229
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
eIF4E4-PA
FBpp0076672
26.4
229
4.79
Q9VRY0
NP_648052
AAF50651
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF4E4 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

5.01

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of eukaryotic translation initiation factor 4F complex
inferred from physical interaction with FLYBASE:eIF4G1; FB:FBgn0023213
(Hernandez et al., 2005)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\eIF4E4 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 0 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of eIF4E4
Transgenic constructs containing regulatory region of eIF4E4
Aberrations (Deficiencies and Duplications) ( 3 )
Inferred from experimentation ( 3 )
Gene disrupted in
Df(3L)ED212
(Marygold et al., 2007)
Df(3L)v65c
(Marygold et al., 2007)
Df(3L)ZN47
(Marygold et al., 2007)
Inferred from location ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (4)
Hsap\EIF4E
14 of 14
Yes
Yes
Hsap\EIF4E1B
13 of 14
No
Yes
Hsap\EIF4E2
5 of 14
No
No
1  
Hsap\EIF4E3
4 of 14
No
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (4)
Rnor\Eif4e
13 of 14
Yes
Yes
Rnor\Eif4e2
4 of 14
No
No
Rnor\Eif4e3
4 of 14
No
Yes
Rnor\Eif4e1b
1 of 14
No
Yes
Mus musculus (laboratory mouse) (4)
Mmus\Eif4e
14 of 14
Yes
Yes
Mmus\Eif4e1b
13 of 14
No
Yes
Mmus\Eif4e2
4 of 14
No
No
Mmus\Eif4e3
4 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (8)
Xtro\eif4e
11 of 13
Yes
Yes
Xtro\eif4e1b
10 of 13
No
Yes
Xtro\eif4e3
2 of 13
No
Yes
Xtro\eif4e2
1 of 13
No
No
Xtro\ganc
1 of 13
No
No
Xtro\kirrel2
1 of 13
No
No
Xtro\mark2
1 of 13
No
No
Xtro\MGC89871
1 of 13
No
No
Danio rerio (Zebrafish) (7)
Drer\eif4ea
14 of 14
Yes
Yes
Drer\eif4eb
14 of 14
Yes
Yes
Drer\eif4e1b
13 of 14
No
Yes
Drer\eif4e1c
13 of 14
No
Yes
Drer\eif4e2
5 of 14
No
No
Drer\eif4e2rs1
4 of 14
No
No
Drer\eif4e3
4 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (5)
Cele\ife-3
13 of 14
Yes
Yes
Cele\ife-5
8 of 14
No
Yes
Cele\ife-1
7 of 14
No
Yes
Cele\ife-2
7 of 14
No
Yes
Cele\ife-4
4 of 14
No
No
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP007172
11 of 12
Yes
Yes
Agam\AgaP_AGAP002948
4 of 12
No
No
Arabidopsis thaliana (thale-cress) (5)
Atha\EIF4E
13 of 13
Yes
Yes
Atha\AT1G29550
12 of 13
No
Yes
Atha\eIF4E3
12 of 13
No
Yes
Atha\LSP1
12 of 13
No
Yes
Atha\NCBP
4 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\CDC33
12 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\tif45
11 of 12
Yes
Yes
Spom\tif452
11 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:eIF4E4. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (6)
eIF4E1
12 of 13
eIF4E3
12 of 13
eIF4E5
12 of 13
eIF4E7
11 of 13
eIF4E6
10 of 13
eIF4EHP
5 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    eIF4E4KK105485
    model of  candidiasis
    CEA
    (Glittenberg et al., 2022)
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    EIF4E; eukaryotic translation initiation factor 4E
    14 of 14
    133440
      EIF4E1B; eukaryotic translation initiation factor 4E family member 1B
      13 of 14
          EIF4E2; eukaryotic translation initiation factor 4E family member 2
          5 of 14
          605895
               
              EIF4E3; eukaryotic translation initiation factor 4E family member 3
              4 of 14
              609896
                  Functional Complementation Data
                  Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                  Interactions
                  Summary of Physical Interactions
                  Summary of Genetic Interactions
                  Interaction Browsers
                  Starting gene(s)
                  Interaction type
                  Interacting gene(s)
                  Reference
                  Starting gene(s)
                  Interaction type
                  Interacting gene(s)
                  Reference
                  External Data
                  Linkouts
                  DroID - A comprehensive database of gene and protein interactions.
                  MIST (protein-protein) - An integrated Molecular Interaction Database
                  Pathways
                  Class of Gene
                  Genomic Location and Detailed Mapping Data
                  Chromosome (arm)
                  3L
                  Recombination map
                  3-17
                  Cytogenetic map
                  65C1-65C1
                  Sequence location
                  FlyBase Computed Cytological Location
                  Cytogenetic map
                  Evidence for location
                  65C1-65C1
                  Limits computationally determined from genome sequence between P{PZ}l(3)0209402094 and P{lacW}l(3)L4060L4060
                  Experimentally Determined Cytological Location
                  Cytogenetic map
                  Notes
                  References
                  Experimentally Determined Recombination Data
                  Location

                  3-17

                  (FlyBase, 2016)

                  3-15.2

                  (Comeron et al., 2012)
                  Left of (cM)
                  Right of (cM)
                  Notes
                  Stocks and Reagents
                  Stocks (62)
                  Genomic Clones (25)
                  cDNA Clones (29)
                   

                  Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                  cDNA clones, fully sequenced
                  BDGP DGC clones
                  Other clones
                  Drosophila Genomics Resource Center cDNA clones

                  For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                  cDNA Clones, End Sequenced (ESTs)
                  BDGP DGC clones
                  Other clones
                  RNAi and Array Information
                  Linkouts
                  DRSC - Results frm RNAi screens
                  Antibody Information
                  Laboratory Generated Antibodies
                   
                  Commercially Available Antibodies
                   
                  Cell Line Information
                  Publicly Available Cell Lines
                   
                    Other Stable Cell Lines
                     
                      Other Comments
                      Relationship to Other Genes
                      Source for database merge of

                      Source for merge of: CG10124 BEST:GH01027

                      (Rana, 2004.7.8)

                      Source for merge of: CG10124 NEST:bs04e06

                      (FlyBase, 1996-)
                      Additional comments

                      Source for merge of CG10124 NEST:bs04e06 was sequence comparison ( date:030903 ).

                      (FlyBase, 1996-)
                      Nomenclature History
                      Source for database identify of

                      Source for identity of: eIF4E-4 CG10124

                      (Hernandez et al., 2005)

                      Source for identity of: eIF4E4 eIF4E-4

                      (Marygold et al., 2017)
                      Nomenclature comments
                      Etymology
                      Synonyms and Secondary IDs (9)
                      Datasets (0)
                      Study focus (0)
                      Experimental Role
                      Project
                      Project Type
                      Title
                      Study result (0)
                      Result
                      Result Type
                      Title
                      External Crossreferences and Linkouts ( 43 )
                      Sequence Crossreferences
                      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                      Other crossreferences
                      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                      FlyMine - An integrated database for Drosophila genomics
                      InterPro - A database of protein families, domains and functional sites
                      KEGG Genes - Molecular building blocks of life in the genomic space.
                      MARRVEL_MODEL - MARRVEL (model organism gene)
                      Linkouts
                      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                      DroID - A comprehensive database of gene and protein interactions.
                      DRSC - Results frm RNAi screens
                      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                      Flygut - An atlas of the Drosophila adult midgut
                      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                      KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                      MIST (protein-protein) - An integrated Molecular Interaction Database
                      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                      References (43)