Scw
HA tag
Tsg
His tag
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
dpp, scw and tsg were cotransfected and purified from conditioned medium.
scw
FLAG tag
tsg
I40E; coordinates relative to tsg-PA
dpp-scw heterodimers were immobilized on anti-Flag beads and the amount of bound tsg was visualized by Western blotting. Addition of transfected sog increased bound tsg.
