FlyBase Gene Report: Dmel\scw

General Information

Symbol

Dmel\scw

Species

D. melanogaster

Name

screw

Annotation Symbol

CG31695

Feature Type

protein_coding_gene

FlyBase ID

FBgn0005590

Gene Model Status

Current

Stock Availability

24 publicly available

Gene Summary

screw (scw) encodes a BMP-type ligand that forms a heterodimer with the product of dpp. The heterodimer is required to achieve the peak levels of BMP signal in the dorsal-most cells of the early blastoderm embryo that are necessary to specify the amnioserosa. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

l(2)37Ff

Key Links

Genomic Location

Cytogenetic map

38A1-38A1

Sequence location

2L:19,699,379..19,700,968 [-]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:19699379..19700968

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein heterodimerization activity

inferred from physical interaction with UniProtKB:P07713

(Shimmi et al., 2005)

inferred from physical interaction with FLYBASE:dpp; FB:FBgn0000490

(Künnapuu et al., 2014)

enables receptor ligand activity

inferred from direct assay

(Shimmi et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables cytokine activity

inferred from biological aspect of ancestor with PANTHER:PTN000218063

(GO Reference Genome Project, 2011-)

enables growth factor activity

inferred from electronic annotation with InterPro:IPR001839, InterPro:IPR017948

(InterPro Project Members, 2004-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in amnioserosa formation

inferred from mutant phenotype

(Nguyen et al., 1998, Arora et al., 1994)

involved_in BMP signaling pathway

inferred from genetic interaction with FLYBASE:dpp; FB:FBgn0000490

(Raftery et al., 1995, Arora et al., 1994)

inferred from genetic interaction with FLYBASE:sax; FB:FBgn0003317,FLYBASE:tkv; FB:FBgn0003716

(Nguyen et al., 1998)

inferred from direct assay

(Künnapuu et al., 2014, Shimmi et al., 2005)

involved_in digestive tract mesoderm development

inferred from mutant phenotype

(Weyers et al., 2011)

involved_in dorsal/ventral pattern formation

inferred from mutant phenotype

(Arora et al., 1994)

involved_in gonad development

inferred from mutant phenotype

(Weyers et al., 2011)

involved_in imaginal disc-derived wing morphogenesis

inferred from mutant phenotype

(Nguyen et al., 1998)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in BMP signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN008528320

(GO Reference Genome Project, 2011-)

involved_in dorsal/ventral pattern formation

traceable author statement

(Raftery and Sutherland, 2003)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in extracellular space

inferred from direct assay

(Künnapuu et al., 2014)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in extracellular region

inferred from electronic annotation with InterPro:IPR015615

(InterPro Project Members, 2004-)

is_active_in extracellular space

inferred from biological aspect of ancestor with PANTHER:PTN000218063

(GO Reference Genome Project, 2011-)

located_in extracellular space

inferred from sequence or structural similarity with FLYBASE:dpp; FB:FBgn0000490

(Arora et al., 1994)

Gene Group (FlyBase)

BONE MORPHOGENETIC PROTEINS

Pathway (FlyBase)

BMP SIGNALING PATHWAY CORE COMPONENTS

Protein Family (UniProt)

Belongs to the TGF-beta family. (P54631)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

BONE MORPHOGENETIC PROTEINS -: Bone Morphogenetic Proteins (BMPs) are a subfamily of the Transforming Growth Factor (TGF)-β family - a group of extracellular ligands for the TGF-β-Receptor family. The binding of a BMP dimer to a heterodimeric serine/threonine kinase receptor complex results in the phosphorylation of Mad, a member of the Smad family. Mad forms a complex with the co-Smad, Med. This complex translocates into the nucleus and regulates the transcription of target genes in concert with other nuclear cofactors. (Adapted from FBrf0236482.)

Pathway (FlyBase)

BMP SIGNALING PATHWAY CORE COMPONENTS -: The Bone Morphogenetic Protein (BMP) signaling pathway is one of two branches of Transforming Growth Factor-β family signaling in Drosophila. The binding of a BMP family dimer to a heterodimeric serine/threonine kinase receptor complex results in the phosphorylation of Mad, a member of the Smad family. Mad forms a complex with the co-Smad, Med. This complex translocates into the nucleus and regulates the transcription of target genes in concert with other nuclear cofactors. (Adapted from FBrf0236482.)

Protein Function (UniProtKB)

Part of the signal that specifies dorsal cell fates in the embryo. Acts together with dpp.

(UniProt, P54631)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

scw: screw

Embyonic lethal; partially ventralized; cephalic furrow shifted dorsally with defects in germ band extension. Ventral denticles extended laterally; spiracles and two to three terminal segments retracted into embryo (Arora and Nusslein-Volhard).

Summary (Interactive Fly)

TGF-ß superfamily - the Scw/dpp heterodimer is required to achieve the peak levels of BMP signal in the dorsal-most cells of the early blastoderm embryo that are necessary to specify the amnioserosa.

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\scw for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P54631)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

scw-RA

FBtr0081261

NM_080124

1342

400

scw-RB

FBtr0343860

NM_001299159

1590

400

Additional Transcript Data and Comments

Reported size (kB)

1.7, 1.4 (northern blot)

(Arora et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

scw-PA

FBpp0080802

45.9

400

8.21

FBpp0310402

45.9

400

8.21

P54631

NP_001286088

AHN54602

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

400 aa isoforms: scw-PA, scw-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

400 (aa); 44 (kD predicted)

(Arora et al., 1994)

Comments

External Data

Subunit Structure (UniProtKB)

Heterodimers of scw/dpp are the active subunit, dpp/dpp homodimers elicit a basal response and scw/scw homodimers alone are ineffective in specifying a dorsal pattern.

(UniProt, P54631)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\scw using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

scw_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

central brain anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism

Comment: rapidly degraded

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Arora et al., 1994)

embryonic stage 4

(Arora et al., 1994)

embryonic stage 5 -- 9

Comment: reference states 2-4 hr AEL

(Arora et al., 1994)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

scw protein is secreted.

(Arora et al., 1994)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular space

inferred from direct assay

(Künnapuu et al., 2014)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\scw in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\scw

View exonic expression by tissue for Dmel\scw

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0005590

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

46000/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0005590

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0005590

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0005590

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31695

Flygut - An atlas of the Drosophila adult midgut

FBgn0005590

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\scw

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 18 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 41 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of scw

Transgenic constructs containing regulatory region of scw

Aberrations (Deficiencies and Duplications) ( 27 )

Inferred from experimentation ( 27 )

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage

lethal - all die during embryonic stage | recessive

partially lethal - majority die, with Scer\GAL4^pnr-MD237

scw^GD10334

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

scw^GD10334

viable, with Scer\GAL4^Mef2.PR

scw^GD10334

Other Phenotypes

Allele

abnormal circadian rhythm, with Scer\GAL4^P2.4.Pdf

scw^KK109520

abnormal circadian rhythm | heat sensitive, with Scer\GAL4^tim.PE, Scer\GAL80^Pdf.PS

scw^KK109520

some die during embryonic stage

some die during embryonic stage | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

scw^GD10334

visible, with Scer\GAL4^A9

scw^UAS.cNa

wild-type

Phenotype manifest in

Allele

abdomen

scw²

abdominal 1 ventral denticle belt

scw⁵

abdominal 2 ventral denticle belt

scw⁵

abdominal 3 ventral denticle belt

scw⁵

abdominal 4 ventral denticle belt

scw⁵

abdominal 5 ventral denticle belt

scw⁵

abdominal 6 ventral denticle belt

scw⁵

abdominal 7 ventral denticle belt

scw⁵

amnioserosa

cephalic furrow

scw^unspecified

cephalopharyngeal skeleton | embryonic stage

scw²

dorsal ectoderm (with scw^E1)

scw^E1R1

dorsal ectoderm (with scw^E1R1)

scw^E1

embryo | dorsal

scw^unspecified

embryo | embryonic stage 14 (with scw^E1)

scw^E1R1

embryo | embryonic stage 14 (with scw^E1R1)

scw^E1

embryo | posterior

scw^unspecified

embryonic/larval crystal cell

scw^l1

embryonic/larval dorsal vessel

scw⁵

embryonic/larval posterior spiracle

scw^E2

embryonic/larval spiracle

scw^unspecified

embryonic leading edge cell

scw^l1

filzkorper

scw²

filzkorper | embryonic stage

scw⁵

gonad | embryonic stage (with scw^8.48)

scw^62.25

gonad | embryonic stage (with scw^62.25)

scw^8.48

mitotic domain 1 | extended germ band stage

ventral denticle belt

scw^unspecified

visceral trunk mesoderm

scw⁵

visceral trunk mesoderm | embryonic stage (with scw^8.48)

scw^62.25

visceral trunk mesoderm | embryonic stage (with scw^62.25)

scw^8.48

wing, with Scer\GAL4^A9

scw^UAS.cNa

wing | distal, with Scer\GAL4^A9

scw^UAS.cNa

wing blade posterior compartment, with Scer\GAL4^A9

scw^UAS.cNa

wing vein, with Scer\GAL4^A9

scw^UAS.cNa

wing vein | ectopic, with Scer\GAL4^A9

scw^UAS.cNa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (33)

Hsap\BMP5

7 of 14

Yes

Hsap\BMP6

7 of 14

Yes

Hsap\BMP7

7 of 14

Yes

Hsap\BMP8A

7 of 14

Yes

Hsap\BMP8B

7 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

Yes

Hsap\BMP15

2 of 14

Yes

Hsap\GDF1

2 of 14

Yes

Hsap\GDF2

2 of 14

Yes

Hsap\GDF3

2 of 14

Yes

Hsap\GDF5

2 of 14

Yes

Hsap\GDF6

2 of 14

Yes

Hsap\GDF7

2 of 14

Yes

Hsap\GDF9

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (32)

Rnor\Bmp6

7 of 14

Yes

Rnor\Bmp8a

7 of 14

Yes

Rnor\Bmp8b

7 of 14

Yes

6 of 14

6 of 14

3 of 14

3 of 14

2 of 14

2 of 14

Yes

Rnor\Bmp15

2 of 14

Yes

Rnor\Gdf1

2 of 14

Yes

Rnor\Gdf3

2 of 14

Yes

Rnor\Gdf5

2 of 14

Yes

Rnor\Gdf6

2 of 14

Yes

Rnor\Gdf7

2 of 14

Yes

Rnor\Gdf9

2 of 14

Yes

Rnor\Gdf11

2 of 14

Rnor\Gdf15

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Rnor\Gdf2

1 of 14

Yes

Rnor\Gdf10

1 of 14

Rnor\Inhbb

1 of 14

Mus musculus (laboratory mouse) (33)

Mmus\Bmp5

7 of 14

Yes

Mmus\Bmp6

7 of 14

Yes

Mmus\Bmp8a

7 of 14

Yes

Mmus\Bmp8b

7 of 14

Yes

6 of 14

3 of 14

3 of 14

2 of 14

2 of 14

Yes

Mmus\Bmp15

2 of 14

Yes

Mmus\Gdf2

2 of 14

Yes

Mmus\Gdf3

2 of 14

Yes

Mmus\Gdf5

2 of 14

Yes

Mmus\Gdf6

2 of 14

Yes

Mmus\Gdf7

2 of 14

Yes

Mmus\Gdf9

2 of 14

Yes

Mmus\Gdf10

2 of 14

Mmus\Gdf11

2 of 14

Mmus\Gdf15

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Mmus\Lefty2

1 of 14

Xenopus tropicalis (Western clawed frog) (41)

Xtro\bmp5

6 of 13

Yes

Xtro\bmp6

6 of 13

Yes

Xtro\bmp7.2

6 of 13

Yes

Xtro\bmp8

6 of 13

Yes

4 of 13

2 of 13

2 of 13

1 of 13

Yes

Xtro\admp

1 of 13

Yes

Xtro\arpin

1 of 13

Yes

Xtro\artn

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\gdf2

1 of 13

Yes

Xtro\gdf3

1 of 13

Yes

Xtro\gdf5

1 of 13

Yes

Xtro\gdf6

1 of 13

Yes

Xtro\gdf7

1 of 13

Yes

Xtro\gdf9

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC101734157

1 of 13

Yes

Xtro\LOC101734231

1 of 13

Yes

Xtro\mreg

1 of 13

Yes

Xtro\mstn.1

1 of 13

Xtro\nid1

1 of 13

Xtro\nodal2

1 of 13

Yes

Xtro\nodal3.1

1 of 13

Yes

Xtro\nodal3.2

1 of 13

Yes

Xtro\nodal5

1 of 13

Yes

Xtro\nodal

1 of 13

Yes

Xtro\nrtn

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (43)

Drer\bmp6

7 of 14

Yes

Drer\bmp7a

7 of 14

Yes

Drer\bmp7b

7 of 14

Yes

Drer\bmp8a

7 of 14

Yes

6 of 14

3 of 14

3 of 14

3 of 14

2 of 14

Yes

Drer\bmp3

2 of 14

Drer\bmp10

2 of 14

Yes

Drer\bmp16

2 of 14

Drer\gdf2

2 of 14

Yes

Drer\gdf3

2 of 14

Yes

Drer\gdf5

2 of 14

Yes

Drer\gdf6a

2 of 14

Yes

Drer\gdf6b

2 of 14

Yes

Drer\gdf7

2 of 14

Yes

Drer\gdf9

2 of 14

Yes

Drer\gdf10a

2 of 14

Drer\gdf10b

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\spaw

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Drer\bmp10l

1 of 14

Yes

Drer\bmp15

1 of 14

Yes

Drer\LOC100329520

1 of 14

Drer\LOC100332902

1 of 14

Drer\ndr2

1 of 14

Yes

Drer\tgfb1b

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\tig-2

6 of 14

Yes

4 of 14

2 of 14

2 of 14

2 of 14

Anopheles gambiae (African malaria mosquito) (6)

Agam\AgaP_AGAP011934

6 of 12

Yes

5 of 12

3 of 12

2 of 12

2 of 12

2 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:scw. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (6)

gbb

8 of 13

dpp

6 of 13

daw

4 of 13

mav

4 of 13

Actβ

3 of 13

myo

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

BMP5; bone morphogenetic protein 5

7 of 14

112265

BMP6; bone morphogenetic protein 6

7 of 14

112266

IRON OVERLOAD, SUSCEPTIBILITY TO; IO

BMP7; bone morphogenetic protein 7

7 of 14

BONE MORPHOGENETIC PROTEIN 7; BMP7

BMP8A; bone morphogenetic protein 8a

7 of 14

620847

BMP8B; bone morphogenetic protein 8b

7 of 14

602284

BMP2; bone morphogenetic protein 2

3 of 14

112261

BMP4; bone morphogenetic protein 4

3 of 14

112262

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

scw

anti tag coimmunoprecipitation, anti tag western blot

(Künnapuu et al., 2014)

scw - dpp

anti tag coimmunoprecipitation, anti tag western blot, comigration in non denaturing gel electrophoresis, western blot

(Malinauskas et al., 2024, Tauscher et al., 2016, Künnapuu et al., 2014, Shimmi et al., 2005)

scw - sog

coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation

(Malinauskas et al., 2024, Negreiros et al., 2018, Shimmi et al., 2005)

scw - tsg

anti tag coimmunoprecipitation, anti tag western blot

(Malinauskas et al., 2024, Shimmi et al., 2005)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

scw

suppressible

sax

(Nguyen et al., 1998)

scw

suppressible

sog

(Nguyen et al., 1998)

scw

suppressible

tkv

(Nguyen et al., 1998)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

dpp

enhanceable

scw

(Raftery et al., 1995)

gbb

suppressible

scw

(Tauscher et al., 2016, Fritsch et al., 2010)

External Data

Subunit Structure (UniProtKB)

Heterodimers of scw/dpp are the active subunit, dpp/dpp homodimers elicit a basal response and scw/scw homodimers alone are ineffective in specifying a dorsal pattern.

(UniProt, P54631 )

Linkouts

BioGRID - A database of protein and genetic interactions.

70052

DroID - A comprehensive database of gene and protein interactions.

FBgn0005590

MIST (genetic) - An integrated Molecular Interaction Database

46000

MIST (protein-protein) - An integrated Molecular Interaction Database

46000

Pathways

Signaling Pathways (FlyBase)

BMP SIGNALING PATHWAY CORE COMPONENTS

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P54631

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-54

Cytogenetic map

38A1-38A1

Sequence location

2L:19,699,379..19,700,968 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

38A1-38A1

Limits computationally determined from genome sequence between P{EP}EP623&P{PZ}spi⁰¹⁰⁶⁸ and P{lacW}l(2)k10239^k10239

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-54

(FlyBase, 2016)

2-53.7

(Comeron et al., 2012)

2-53

(Tearle and Nusslein-Volhard, 1987)

Left of (cM)

Right of (cM)

Notes

scw^E1 and scw^E2 are located 0.08cM apart.

(Raftery et al., 1995)

Stocks and Reagents

Stocks (24)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

606463

w¹¹¹⁸; P{scw.HA}2

606398

w¹¹¹⁸; P{UAS-scw.M.HA}3

64098

y¹ w^*; P{scw-YFP.R}24.1

63061

y¹ w^*; P{UAS-scw.R}1/CyO

63062

P{scw-YFP.R}23.1, y¹ w^*/FM7h

4351

scw^l1 rdo¹ hook¹ pr¹/CyO

63060

y¹ w^*; P{UAS-scw.R}51/TM3, Sb¹ Ser¹

Kyoto

107700

scw^l1 rdo¹ hook¹ pr¹/CyO

31695R-1

31695R-2

v105303

v21400

w¹¹¹⁸; P{GD10334}v21400

More stocks available...

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD30945

Other clones

DPiM_LD30945

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0005590

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0005590

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The scw product negatively regulates dac expression in the embryonic head.

(Anderson et al., 2006)

New annotation (CG31695) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

dpp/tkv signaling is obligatorily required, while scw/sax signaling is necessary but not sufficient for dorsal patterning in the embryo.

(Nguyen et al., 1998)

scw is located between exons 4 and 5 of Lar.

(Krueger et al., 1996)

scw positively modulates dpp activity.

(Arora et al., 1995)

Genetic and phenotypic data reveals that scw is involved in post-transcriptional regulation of dpp activity by elevating dpp activity dorsally. Mutations in scw disrupt amnioserosa formation. Injection of dpp RNA into the ventral side does not result in an asymmetric response, a second amnioserosa forms at the site of injection. This suggests that the scw protein is critical to the local response to dpp RNA.

(Neul and Ferguson, 1995)

scw and dpp act together to establish distinct response boundaries within the dorsal half of the embryo, perhaps by forming heterodimers that have a higher activity than homodimers of either molecule alone.

(Arora et al., 1994)

scw alleles display relative phenotypic strengths, this may be correlated to the progressive loss of dorsal pattern elements in the ventralised mutants.

(Arora and Nusslein-Volhard, 1992)

scw has been molecularly characterised.

(Arora et al., 1992)

scw is required for the normal ontogeny of the zen pattern and fating of the amnioserosa.

(Ray et al., 1991)

Involved in the regulatory hierarchy responsible for the asymmetric distribution and function of zygotic regulatory gene products along the DV axis of early embryos. scw mutants exhibit a weak ventralising phenotype in which the maintenance of the zen pattern is affected.

(Rushlow and Levine, 1990)

Relationship to Other Genes

Source for database merge of

Source for merge of: scw BcDNA:LD30945

(FlyBase, 1996-)

Additional comments

Source for merge of scw BcDNA:LD30945 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

BcDNA:LD30945

CG31695

(National Institute of Genetics Fly Stocks, 2022-, Hosono et al., 2015, Keleman et al., 2009.8.5, Brandt, 2006)

DmSCW

(Wisotzkey and Newfeld, 2020)

SCW

(Bickel et al., 2008)

Scw

(Bressan and Araujo, 2022, Montanari et al., 2022, Madamanchi et al., 2021, Negreiros et al., 2018, Upadhyay et al., 2017, Matsuda et al., 2016, Tauscher et al., 2016, Bier and De Robertis, 2015, Ting et al., 2014, Mbodj et al., 2013, Sawala et al., 2012, Peluso et al., 2011, Wang et al., 2008, Pistillo and Desplan, 2007, Dudu, 2006, Umulis et al., 2006, Wang and Ferguson, 2004)

l(2)37Ff

(Jones and McGinnis, 1993)

l(2)IG76

mor2

(Weyers et al., 2011)

screw

(Gautam et al., 2021)

scw

(O'Connor, 2025.5.8, Bastin and Eleftherianos, 2023, Lassetter et al., 2023, Pérez-Mojica et al., 2023, Kubrak et al., 2022, National Institute of Genetics Fly Stocks, 2022-, Varga et al., 2022, Rushton et al., 2020, Statzer and Ewald, 2020, Jia et al., 2018, Transgenic RNAi Project members, 2017-, Sandler and Stathopoulos, 2016, Tauscher et al., 2016, Ray, 2015.6.29, Ray, 2015.6.29, Umulis and Othmer, 2015, Deshpande et al., 2014, Künnapuu et al., 2014, Beckwith et al., 2013, Chen et al., 2013, Saunders et al., 2013, Wotton et al., 2013, Fritsch et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Fritsch et al., 2010, Salzer et al., 2010, Umulis et al., 2010, Pentek et al., 2009, Twombly et al., 2009, Liang et al., 2008, Fritsch and Ray, 2007, Junion et al., 2007, Lecuyer et al., 2007, Anderson et al., 2006, Brandt, 2006, Gim et al., 2001)

Name Synonyms

Screw

(Destalminil-Letourneau et al., 2021, Shimmi and Newfeld, 2013, Umulis et al., 2010, Bickel et al., 2008, Wang et al., 2008, Fritsch and Ray, 2007, Stathopoulos and Levine, 2005, Gim et al., 2001)

morphological defects 2

(Weyers et al., 2011)

screw

(Wieschaus and Nüsslein-Volhard, 2016, Heimiller et al., 2014, Künnapuu et al., 2014, Wotton et al., 2013, Weyers et al., 2011, Salzer et al., 2010, Wright et al., 2010, Twombly et al., 2009, Anderson et al., 2006, Arora, 1994.11.23)

Secondary FlyBase IDs

FBgn0002053
FBgn0003343
FBgn0046219
FBgn0051695

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 42 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

46000

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P54631

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P54631

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

X2JB15

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P54631

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0005590

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

46000/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0005590

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0005590

FlyMine - An integrated database for Drosophila genomics

FBgn0005590

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG31695

MARRVEL_MODEL - MARRVEL (model organism gene)

46000

Linkouts

BioGRID - A database of protein and genetic interactions.

70052

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0005590

DroID - A comprehensive database of gene and protein interactions.

FBgn0005590

DRSC - Results frm RNAi screens

FBgn0005590

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

scw_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0005590

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0005590

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31695

Flygut - An atlas of the Drosophila adult midgut

FBgn0005590

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

46000

MIST (protein-protein) - An integrated Molecular Interaction Database

46000

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P54631

References (215)

The following external sites may use different assemblies or annotations than FlyBase.

NA

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