scw, dpp Physical Interaction report

Collection

Source/Stage

Cell line used

comigration in non denaturing gel electrophoresis

Participants

Corresponds to

Reported as

Role

Note

scw

Scw

bait

FLAG tag

dpp

Dpp

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Shimmi et al., 2005)

dpp and scw were cotransfected and purified from conditioned medium.

(Shimmi et al., 2005)

FBrf0183778-2.CM.WB

Reference

Shimmi et al., 2005

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

scw

Scw

bait

FLAG tag

dpp

Dpp

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Shimmi et al., 2005)

Under non-reducing conditions, both dpp and scw comigrate with apparent molecular weight expected for the heterodimer, suggesting the presence of disulfide bridges.

(Shimmi et al., 2005)

dpp and scw were cotransfected and immunoprecipitated from conditioned medium and separated by SDS-PAGE under reducing or non-reducing conditions.

(Shimmi et al., 2005)

FBrf0183778-3.coIP.WB

Reference

Shimmi et al., 2005

Description

physical association

Assay

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

dpp

Dpp

bait

HA tag

scw

Scw

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Shimmi et al., 2005)

FBrf0224640-1.coIP.WB

Reference

Künnapuu et al., 2014

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2-DGRC

Participants

Corresponds to

Reported as

Role

Note

scw

Scw

bait

FLAG tag

scw

dpp

Dpp

prey

HA tag

dpp

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

scw

15kDa mature ligand

sufficient binding region

aa 55-271

"L15" form

scw

prodomain cleavage site

mutation increasing interaction

aa 91

R91C, corresponds to scw[E1] allele

dpp

mature ligand

sufficient binding region

"Dpp-S" form

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

scw-dpp heterodimers were recovered in both cell lysates and conditioned media.

(Künnapuu et al., 2014)

Source was extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Künnapuu et al., 2014)

Mutation of the prodomain cleavage site in the scw[E1] mutation (R91C) increases scw-dpp heterodimerization while decreasing scw homodimerization. This may explain why the scw[E1] mutation enhances dpp hypomorphs.

(Künnapuu et al., 2014)

FBrf0233838-1.coIP.WB

Reference

Tauscher et al., 2016

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

scw

Scw

bait

FLAG tag

scw

dpp

Dpp

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

scw

glycosylation site N304

mutation increasing interaction

aa 304

N304Q, "N1Q" and "N1_N2Q" construct, coordinates relative to scw-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tauscher et al., 2016)

Heterodimers were immunoprecipitated from conditioned medium.

(Tauscher et al., 2016)

FBrf0259693-1.coIP.WB

Reference

Malinauskas et al., 2024

Description

physical association

Assay

Collection

Source/Stage

Cell line used